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- 15:28, 30 June 2024 Richert talk contribs created page UV-Melting Curves (Created page with " '''UV-Melting Points''' One traditional method to determine the stability of DNA or RNA duplexes is to heat an aqueous solution of the duplex in question and observing the change in the UV absorption of the solution as the temperature increases. Upon dissociation of the duplex, the base stacking is lost, so that the UV absorption increases. Plotting the UV absorption against the temperature then yields a so-called melting curve. The temperature at which half of the d...") Tag: Visual edit