UV-Melting Curves: Revision history

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5 August 2024

9 July 2024

2 July 2024

30 June 2024

  • curprev 15:2815:28, 30 June 2024Richert talk contribs 961 bytes +961 Created page with " '''UV-Melting Points''' One traditional method to determine the stability of DNA or RNA duplexes is to heat an aqueous solution of the duplex in question and observing the change in the UV absorption of the solution as the temperature increases. Upon dissociation of the duplex, the base stacking is lost, so that the UV absorption increases. Plotting the UV absorption against the temperature then yields a so-called melting curve. The temperature at which half of the d..." Tag: Visual edit