Quantitative MALDI-TOF MS of Oligonucleotides - Revision history

Dragos: Fixed formatting for "Papers on quantitative MALDI-TOF" sub-heading.

2024-08-05T11:23:19Z

Fixed formatting for "Papers on quantitative MALDI-TOF" sub-heading.

← Older revision		Revision as of 13:23, 5 August 2024
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	[4] MALDI MS. A Practical Guide to Instrumentation, Methods and Applications. Edited by Franz Hillenkamp and Jasna Peter-Katalinic, Wiley-VCH Verlag, Weinheim, 2007. ISBN: 978-3-527-31440-9		[4] MALDI MS. A Practical Guide to Instrumentation, Methods and Applications. Edited by Franz Hillenkamp and Jasna Peter-Katalinic, Wiley-VCH Verlag, Weinheim, 2007. ISBN: 978-3-527-31440-9

	==== Papers on quantitative MALDI-TOF MS of oligonucleotides (cited by later papers on the topic) ====		=== Papers on quantitative MALDI-TOF MS of oligonucleotides (cited by later papers on the topic) ===
	[5] D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548. https://doi.org/10.1016/0960-894X(96)00465-9		[5] D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548. https://doi.org/10.1016/0960-894X(96)00465-9

Dragos: Fixed the reference numbering.

2024-08-05T11:22:27Z

Fixed the reference numbering.

← Older revision		Revision as of 13:22, 5 August 2024
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	==== Papers on quantitative MALDI-TOF MS of oligonucleotides (cited by later papers on the topic) ====		==== Papers on quantitative MALDI-TOF MS of oligonucleotides (cited by later papers on the topic) ====
	[1] D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548. https://doi.org/10.1016/0960-894X(96)00465-9		[5] D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548. https://doi.org/10.1016/0960-894X(96)00465-9

	[2] K. Berlin, R. K. Jain, C. Tetzlaff, C. Steinbeck, C. Richert, Spectrometrically monitored selection experiments: quantitative laser desorption mass spectrometry of small chemical libraries. ''Chem. Biol.'' '''1997''', ''4'', 63-77. https://doi.org/10.1016/s1074-5521(97)90237-4		[6] K. Berlin, R. K. Jain, C. Tetzlaff, C. Steinbeck, C. Richert, Spectrometrically monitored selection experiments: quantitative laser desorption mass spectrometry of small chemical libraries. ''Chem. Biol.'' '''1997''', ''4'', 63-77. https://doi.org/10.1016/s1074-5521(97)90237-4

	[3] J. Störker, J. Mayo, C. N. Tetzlaff, D. A. Sarracino, I. Schwope, C. Richert, Rapid genotyping via MALDI-monitored nuclease selection from probe libraries, ''Nature Biotechn.'' '''2000''', ''18'', 1213-1216. https://doi.org/10.1038/81226		[7] J. Störker, J. Mayo, C. N. Tetzlaff, D. A. Sarracino, I. Schwope, C. Richert, Rapid genotyping via MALDI-monitored nuclease selection from probe libraries, ''Nature Biotechn.'' '''2000''', ''18'', 1213-1216. https://doi.org/10.1038/81226

Dragos: Fixed a typo, cleaned up the page formatting, redid the numbering of the references and added links to the cited papers.

2024-08-05T10:52:14Z

Fixed a typo, cleaned up the page formatting, redid the numbering of the references and added links to the cited papers.

← Older revision		Revision as of 12:52, 5 August 2024
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	~~'''~~Quantitative MALDI-TOF Mass Spectrometry of Oligonucleotides~~'''~~		== Quantitative MALDI-TOF Mass Spectrometry of Oligonucleotides ==


	The acronym MALDI-TOF MS stands for Matrix-Assisted Laser-Desorption-Ionization Time-of-Flight Mass Spectrometry. This type of mass spectrometry was invented by Franz Hillenkamp and Michael Karas at the University of Münster.		The acronym MALDI-TOF MS stands for Matrix-Assisted Laser-Desorption-Ionization Time-of-Flight Mass Spectrometry. This type of mass spectrometry was invented by Franz Hillenkamp and Michael Karas at the University of Münster.



	The advantage of MALDI MS is that it gives a sharp, predictable signal for a biomacromolecule, with great sensitivity and robustness to assay components like buffers. One disadvantage is the difficult to quantify. One approach to overcome this weakness is to use an internal standard or relative signal intensity, proper acquisition conditions, calibration plots and correction factors. Acquisition conditions that have been found to be successful include using moderately increased laser power and at least 100 laser shots to achieve near-exhaustive ablation of a given region of the matrix, acquiring several spectra and averaging over the relative signal (analyte versus internal standard) to obtain reliable data on concentration. Some matrices give more reproducible spectra than others. For example, trihydroxyacetophenone with diammonium citrate as co-matrix fulfills these criteria and has been used to monitor selection, genotyping or footprinting assays.		The advantage of MALDI MS is that it gives a sharp, predictable signal for a biomacromolecule, with great sensitivity and robustness to assay components like buffers. One disadvantage is the difficult to quantify. One approach to overcome this weakness is to use an internal standard or relative signal intensity, proper acquisition conditions, calibration plots and correction factors. Acquisition conditions that have been found to be successful include using moderately increased laser power and at least 100 laser shots to achieve near-exhaustive ablation of a given region of the matrix, acquiring several spectra and averaging over the relative signal (analyte versus internal standard) to obtain reliable data on concentration. Some matrices give more reproducible spectra than others. For example, trihydroxyacetophenone with diammonium citrate as co-matrix fulfills these criteria and has been used to monitor selection, genotyping or footprinting assays.

			== References ==

			=== MALDI Papers ===
			[1] M. Karas, F. Hillenkamp, Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. ''Anal. Chem.'' '''1988''', ''60'', 2299–2301. https://doi.org/10.1021/ac00171a028

	~~'''References'''~~		[2] Berkenkamp, S.; Kirpekar, F.; Hillenkamp, F. Infrared MALDI mass spectrometry of large nucleic acids. ''Science'' '''1998''', ''281'', 260-262. https://doi.org/10.1126/science.281.5374.260


	~~''MALDI Papers''~~

	~~a) M. Karas, F. Hillenkamp, Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. ''Anal. Chem.'' '''1988''', ''60'', 2299–2301.~~

	~~b) Berkenkamp~~, S.; Kirpekar, F.; Hillenkamp, F. Infrared MALDI mass spectrometry of large nucleic acids. ''Science'' '''1998''', ''281'', 260-262.

	~~c) Kirpekar, F.; Nordhoff, E~~.~~; Larsen, L~~. K.~~; Kristiansen, K~~.~~; Roepstorff, P.; Hillenkamp, F. DNA sequence analysis by MALDI mass spectrometry. ''Nucleic Acids Res.'' '''1998''', ''26'', 2554-2559~~.

	~~d) MALDI MS~~. ~~A Practical Guide to Instrumentation~~, ~~Methods and Applications~~. ~~Edited by Franz~~ Hillenkamp ~~and Jasna Peter-Katalinic~~, ~~Wiley-VCH Verlag~~, ~~Weinheim~~, ~~2007~~. ~~ISBN~~: ~~978-3-527-31440-9~~		[3] Kirpekar, F.; Nordhoff, E.; Larsen, L. K.; Kristiansen, K.; Roepstorff, P.; Hillenkamp, F. DNA sequence analysis by MALDI mass spectrometry. ''Nucleic Acids Res.'' '''1998''', ''26'', 2554-2559. https://doi.org/10.1093%2Fnar%2F26.11.2554

	~~''<br />~~		[4] MALDI MS. A Practical Guide to Instrumentation, Methods and Applications. Edited by Franz Hillenkamp and Jasna Peter-Katalinic, Wiley-VCH Verlag, Weinheim, 2007. ISBN: 978-3-527-31440-9
	~~''Paper on quantitative~~ MALDI~~-TOF~~ MS ~~of oligonucleotides (cited~~ by ~~later papers on the topic)''~~

	a) D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548.		==== Papers on quantitative MALDI-TOF MS of oligonucleotides (cited by later papers on the topic) ====
			[1] D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548. https://doi.org/10.1016/0960-894X(96)00465-9

	b) K. Berlin, R. K. Jain, C. Tetzlaff, C. Steinbeck, C. Richert, Spectrometrically monitored selection experiments: quantitative laser desorption mass spectrometry of small chemical libraries. ''Chem. Biol.'' '''1997''', ''4'', 63-77.		[2] K. Berlin, R. K. Jain, C. Tetzlaff, C. Steinbeck, C. Richert, Spectrometrically monitored selection experiments: quantitative laser desorption mass spectrometry of small chemical libraries. ''Chem. Biol.'' '''1997''', ''4'', 63-77. https://doi.org/10.1016/s1074-5521(97)90237-4

	c) J. Störker, J. Mayo, C. N. Tetzlaff, D. A. Sarracino, I. Schwope, C. Richert, Rapid genotyping via MALDI-monitored nuclease selection from probe libraries, ''Nature Biotechn.'' '''2000''', ''18'', 1213-1216.		[3] J. Störker, J. Mayo, C. N. Tetzlaff, D. A. Sarracino, I. Schwope, C. Richert, Rapid genotyping via MALDI-monitored nuclease selection from probe libraries, ''Nature Biotechn.'' '''2000''', ''18'', 1213-1216. https://doi.org/10.1038/81226

Richert at 16:42, 29 June 2024

2024-06-29T16:42:01Z

← Older revision		Revision as of 18:42, 29 June 2024
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	The advantage of MALDI MS is that it gives a sharp, predictable signal for a biomacromolecule, with great sensitivity and robustness to assay components like buffers. One disadvantage is the difficult to quantify. One approach to overcome this weakness is to use an internal standard or relative signal intensity, proper acquisition conditions, calibration plots and correction factors.
			The advantage of MALDI MS is that it gives a sharp, predictable signal for a biomacromolecule, with great sensitivity and robustness to assay components like buffers. One disadvantage is the difficult to quantify. One approach to overcome this weakness is to use an internal standard or relative signal intensity, proper acquisition conditions, calibration plots and correction factors. Acquisition conditions that have been found to be successful include using moderately increased laser power and at least 100 laser shots to achieve near-exhaustive ablation of a given region of the matrix, acquiring several spectra and averaging over the relative signal (analyte versus internal standard) to obtain reliable data on concentration. Some matrices give more reproducible spectra than others. For example, trihydroxyacetophenone with diammonium citrate as co-matrix fulfills these criteria and has been used to monitor selection, genotyping or footprinting assays.



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	''Paper on quantitative MALDI-TOF MS of oligonucleotides (cited by later papers on the topic)''		''Paper on quantitative MALDI-TOF MS of oligonucleotides (cited by later papers on the topic)''

	D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548.		a) D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548.

			b) K. Berlin, R. K. Jain, C. Tetzlaff, C. Steinbeck, C. Richert, Spectrometrically monitored selection experiments: quantitative laser desorption mass spectrometry of small chemical libraries. ''Chem. Biol.'' '''1997''', ''4'', 63-77.

			c) J. Störker, J. Mayo, C. N. Tetzlaff, D. A. Sarracino, I. Schwope, C. Richert, Rapid genotyping via MALDI-monitored nuclease selection from probe libraries, ''Nature Biotechn.'' '''2000''', ''18'', 1213-1216.

Richert at 09:08, 25 June 2024

2024-06-25T09:08:26Z

← Older revision		Revision as of 11:08, 25 June 2024
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	''<br />		''<br />
	Paper on quantitative MALDI-TOF MS of oligonucleotides (cited by later papers on the topic)''		''Paper on quantitative MALDI-TOF MS of oligonucleotides (cited by later papers on the topic)''

	D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548.		D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548.

Richert at 09:07, 25 June 2024

2024-06-25T09:07:38Z

← Older revision		Revision as of 11:07, 25 June 2024
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	d) MALDI MS. A Practical Guide to Instrumentation, Methods and Applications. Edited by Franz Hillenkamp and Jasna Peter-Katalinic, Wiley-VCH Verlag, Weinheim, 2007. ISBN: 978-3-527-31440-9		d) MALDI MS. A Practical Guide to Instrumentation, Methods and Applications. Edited by Franz Hillenkamp and Jasna Peter-Katalinic, Wiley-VCH Verlag, Weinheim, 2007. ISBN: 978-3-527-31440-9

			''<br />
	Paper on quantitative MALDI-TOF MS of oligonucleotides (cited by later papers on the topic)		Paper on quantitative MALDI-TOF MS of oligonucleotides (cited by later papers on the topic)''

	D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548.		D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548.

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2024-06-23T06:03:54Z

Created page with "'''Quantitative MALDI-TOF Mass Spectrometry of Oligonucleotides''' The acronym MALDI-TOF MS stands for Matrix-Assisted Laser-Desorption-Ionization Time-of-Flight Mass Spectrometry. This type of mass spectrometry was invented by Franz Hillenkamp and Michael Karas at the University of Münster. The advantage of MALDI MS is that it gives a sharp, predictable signal for a biomacromolecule, with great sensitivity and robustness to assay components like buffers. One disad..."

New page

'''Quantitative MALDI-TOF Mass Spectrometry of Oligonucleotides'''

The acronym MALDI-TOF MS stands for Matrix-Assisted Laser-Desorption-Ionization Time-of-Flight Mass Spectrometry. This type of mass spectrometry was invented by Franz Hillenkamp and Michael Karas at the University of Münster.

The advantage of MALDI MS is that it gives a sharp, predictable signal for a biomacromolecule, with great sensitivity and robustness to assay components like buffers. One disadvantage is the difficult to quantify. One approach to overcome this weakness is to use an internal standard or relative signal intensity, proper acquisition conditions, calibration plots and correction factors.

'''References'''

''MALDI Papers''

a) M. Karas, F. Hillenkamp, Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. ''Anal. Chem.'' '''1988''', ''60'', 2299–2301.

b) Berkenkamp, S.; Kirpekar, F.; Hillenkamp, F. Infrared MALDI mass spectrometry of large nucleic acids. ''Science'' '''1998''', ''281'', 260-262.

c) Kirpekar, F.; Nordhoff, E.; Larsen, L. K.; Kristiansen, K.; Roepstorff, P.; Hillenkamp, F. DNA sequence analysis by MALDI mass spectrometry. ''Nucleic Acids Res.'' '''1998''', ''26'', 2554-2559.

d) MALDI MS. A Practical Guide to Instrumentation, Methods and Applications. Edited by Franz Hillenkamp and Jasna Peter-Katalinic, Wiley-VCH Verlag, Weinheim, 2007. ISBN: 978-3-527-31440-9

Paper on quantitative MALDI-TOF MS of oligonucleotides (cited by later papers on the topic)

D. Sarracino, C. Richert, Quantitative MALDI-TOF Spectrometry of Oligonucleotides and a Nuclease Assay. ''Bioorg. Med. Chem. Lett.,'' '''1996''', ''6'', 2543-2548.